CCOGC collaborators determined that an essential resource needed in the field was the development of well-described repository of tissues (tumor and normal) from tumor bearing dogs. To be optimally useful this biospecimen repository would be:
- Focused on specific cancers of interest to the field
- Centrally housed with multiple contributors
- Populated with prospectively collected tissues and fluids
- Annotated with robust parallel clinical data-base
- Managed through a web-enabled entry and retrieval port
- Publicly accessible based on scientific merit or requesting individuals
Based on this, the repository has begun with a goal of collecting tissues and fluids from 3,000 dogs with specific cancer types. The specific cancers selected represent those that are not only major problems in the dog population, but which are also have comparative value in human cancer investigation. Universal and cancer specific standard operative procedures have been defined for the collections. The bank is housed centrally within a contract biospecimen repository in Frederick Maryland with contributors including qualified schools of veterinary medicine in the United States. A caBIG compliant web-based relational database has been implemented to track sample shipment, to describe sample characteristics and clinical annotation of patients, and to allow review of bank assets.
Biospecimen Repository Physical Structure and Procedures
The Pfizer-CCOGC Biospecimen Repository has received support from the intramural National Cancer Institute to establish and maintain a physical bank in Frederick Maryland and to have this bank linked to a web-enabled interface for access to tissues.
The physical bank is managed by Fisher Bioservices and is part of the NCI Frederick Central Repository Services. The facility is over 33,000 sq ft, has 24-hour temperature monitoring, four 250KW generators, and back-up generators. The bank consists of -80C freezers; chemical storage for formalin fixed and paraffin embedded tissues, bar coding hard and soft ware and web enabled sample management system. The bioinformatics platform for the repository includes a relational database that will connect clinical information on samples entered to the study with a front and back end retrieval system. Biological data derived from samples in the Biospecimen Repository are uploaded into the bioinformatics database and become part of the progressive value of the Repository. It is expected that the value of this biological data will exceed the physical value of tissues over time.
The biospecimen repository houses tumor tissue, normal tissues, serum, plasma, peripheral blood mononuclear cell preparations, genomic DNA, RNA and urine samples. The tumor and normal tissue collected and processed in both ethanol and two frozen formats (both flash frozen and OCT embedded). The repository follows many parts of the organization and structure of the proposed National Biospecimen Network Blueprint1. Standard operating procedures of tissue/sample collection have been established.